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@#AIM: To analyze the diagnostic value of Pentacam anterior segment analyzer examination parameters on early keratoconus.<p>METHODS: Retrospective control study. Totally 100 patients(124 eyes)with keratoconus who were treated in the hospital between January 2019 and January 2020 were selected as study group. According to the severity of Amsler-Krumeich classification, patients with keratoconus were divided into mild group(51 eyes), moderate group(43 eyes)and severe group(30 eyes), and another 30 normal people with past history of myopia and astigmatism(30 eyes)were selected as control group. The Pentacam anterior segment analyzer parameters \〖horizontal central curvature of 3mm diameter range(K1), vertical central curvature of 3mm diameter range(K2), maximum refractive power of corneal anterior surface(Kmax), corneal astigmatism(Cyl), corneal thickness at the thinnest(thinnest local), ROC curve was used to analyze the sensitive indicators in the diagnosis of early keratoconus.<p>RESULTS:The K1, K2, Kmax, Cyl, ISV, IVA, KI and ACD in study group were significantly higher than those in control group(<i>P</i><0.05)while the thinnest local and ACV were significantly lower than those in control group(<i>P</i><0.05). There were statistically significant differences in the K1, K2, Kmax, Cyl, thinnest local, ISV, IVA, KI, ACV and ACD among patients with different degrees of keratoconus(<i>P</i><0.05), and the K1, K2, Kmax, Cyl, ISV, IVA, KI and ACD in moderate group and severe group were significantly higher than those in mild group(<i>P</i><0.05)while the thinnest local and ACV were significantly lower than those in mild group(<i>P</i><0.05), and there were significant differences in the parameters between moderate group and severe group(<i>P</i><0.05). ROC diagnostic curve results showed that Kmax, thinnest local, ISV, IVA and KI were sensitive indicators for diagnosing keratoconus(AUC>0.85), of which ISV had the highest diagnostic value.<p>CONCLUSION: Pentacam anterior segment analyzer can effectively measure the keratoconus parameters. There are differences in the parameters among patients with different degrees of keratoconus. Kmax, thinnest local, ISV, IVA and KI are sensitive indicators for diagnosing early keratoconus, and Pentacam anterior segment analyzer examination parameters have a higher diagnostic value on early keratoconus.
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Objective: The content of 16 kinds of organochlorine pesticides including As, Hg, Pb, Cd, and Cu in Tetrastigma hemsleyanum from different places were determined. Methods: The content of As, Hg, Pb, Cd, and Cu in T. hemsleyanum from different places were determined by ICP-MS, and organochlorine pesticides were determined by GC. Results: The contents of heavy metals in T. hemsleyanum were Pb≤4.167 7 mg/kg, Cd≤0.194 6 mg/kg, As≤0.455 0 mg/kg, Hg≤0.042 4 mg/kg, Cu≤7.892 5 mg/kg. Organochlorine preticides were lower in T. hemsleyanum. Conclusion: The method is simple, efficient and accurate, which can be used for the safety evaluation of T. hemsleyanum.
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<p><b>OBJECTIVE</b>To investigate the effect of nifedipine of therapeutic dosage on the plasma membrane functional integrity and osmosensitive calcium influx in human sperm in vitro.</p><p><b>METHODS</b>Sperm samples were aseptically obtained from 10 healthy fertile men by masturbation and prepared by swim-up technique to produce a spermatozoal solution of high motility. The solution was then incubated with nifedipine of 20, 100 and 20 x 10(3) ng/ml respectively at 37 degrees C in vitro. The hypo-osmotic swelling (HOS) test was done to assess the sperm function. Intracellular calcium concentration was measured by fluorescent probe fura-2/AM before and after sperm medium dilution in distilled water.</p><p><b>RESULTS</b>The 20 x 103 ng/ml group showed significantly lower HOS scores than the control (P < 0.01). The 20, 100 and 20 x 10(3) ng/ml groups all showed significantly lower Ca2+ fluorescence D-value than the control (P < 0.01).</p><p><b>CONCLUSION</b>Nifedipine can modify plasma membrane functional integrity and inhibit osmosensitive calcium influx in human sperm and affect male fertility in vitro in therapeutic dose.</p>